Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). Fluorescent dextran ; Picout, D.R. Masuelli, Martin Alberto (2013). Dextran consists of -D glucose units with a majority of (16) glucosidic linkages between them. Based on these results a difference of approximately 1015% between the amounts of drugs permeated through the human skin and pig skin models should be considered during human clinical trials. Morris, G.A. Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. The site is secure. 2015;32(5):170413. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. Antoniou, E.; Buitrago, C. F.; Tsianou, M.; Alexandridis, P. (2010). Anal. De Belder, A. N. (2001). eCollection 2018 Feb. Molecules. Analytes and stationary phase with Universal Calibration Relative Measurement Concentration and MW detector: RI + Viscometer Relationship between hydrodynamic volume (MW*intrinsic viscosity) and elution volume Multiple detection After 24h the micropores were either closed or reduced further to smaller sizes, 37.5020.41, 50.5718.07, and 58.5814.99m, for 2, 5, and 10 times microporation, respectively. ; Hogan, M.P. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. Schematic illustration of transdermal delivery after the application of the microporation device. In this sense, the largest deviations were obtained for the highest molecular weight (580-2000kDa with values from 0.8467 to 0.7346). Small Intestinal Permeability and Gut-Transit Time Determined Conducting a comparative analysis of the data shown in Table 1 can observer that parameter data Mark-Houwink a, obtained by these authors are very similar to that obtained in this work, which affirms its validity. a Microscopy images of micropore and b quantified data using ImageJ after 0 (immediately), 4, 8, and 24h according to the number of times microproration was done (n=150, *: p<0.05). Learn More. The importance of this type of study lies in the analysis of the polysaccharide behavior at industrial processes, the requirements to reduce energy, avoid flow problems and product quality control. Privacy Ahn, G.Y., Eo, HS., Kim, D. et al. AATEX. Biophysical Journal, 87, 4259-4270. Anyone you share the following link with will be able to read this content: Sorry, a shareable link is not currently available for this article. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Dextran. The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. Microporations were performed 2, 5, and 10 times and the skin damage was confirmed using the TEWL measurement. Mitragotri S. Mechanical disruption of skin barrier for vaccine delivery. The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. Carbohydrate Polymers 95 360-365. Using the polymer standards, a plot of the ln [] vs ln Mw usually gives a straight line. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. Measurements were made with a Ubbelohde C type suspended level viscometer [57] (IVA, Argentina); with a diameter capillary of 0.81mm and water draining time of 35.89s at 20C, with viscometer constant of 0.027883 mm2/s [58]. Dulbeccos Phosphate-Buffered Saline (DPBS) was purchased from Welgene (Gyeongsan, Korea). The important role of thermodynamic interaction parameter in the determination of theta temperature, dextran/water system. Hrabchak C, Flynn L, Woodhouse KA. Conversely, fluorescein sodium was the compound selected to model hydrophilic, low MW drugs. Synthesis and characterization of blue dextrans (2008). Carbohydrate Polymers 99, 199-207. Please enable it to take advantage of the complete set of features! 2013;43(4):287303. Structural and biocompatibility properties of dextran from Sung-Wook Choi. Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine WebFITC Products. The effectiveness of the RF microporation technique in transdermal drug delivery of macromolecules and hydrophilic agents such as peptides, hormones, and vaccines has been demonstrated extensively in previous studies [16,17,18]. Structural and biocompatibility properties of dextran from government site. Its Invariancy with Respect to the Cross-Sectional Dimensions of Polymer Chains. Recent Pat. and transmitted securely. The regeneration process of the skin is regulated by growth factors such as keratinocyte growth factor and fibroblast growth factor, which trigger the proliferation and migration of cells [23, 24]. Macromolecules, 31, 7712-7716. 4h after microporation, the sizes of the micropore decreased slightly. Small Intestinal Permeability and Gut-Transit Time Determined International Journal of Biological Macromolecules, 23, 135-141. 4K, b M.W. Format). https://doi.org/10.1186/s40824-020-00201-7, DOI: https://doi.org/10.1186/s40824-020-00201-7. Dextran Specifications: Label (Ex/Em): Fluorescein (494/521) Size: 40,000 MW. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. International Journal of Pharmaceutics, 277, 99-104. (2007). J. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. The genus Weissella: taxonomy, ecology and biotechnological potential. WebThe hydrodynamic radius ( Rh) determined by our Fluidity instruments is the Stokes radius, or the size of a hard spherical particle that diffuses at the same rate as the protein (s) detected. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC dextran measured in plasma 1 h after its oral application is a marker of small intestinal permeability. J. Braz. where tr is the column retention time in minutes (2 of 0.9792). (2013). Protein Hydrodynamics with Light Scattering WebR H Hydrodynamic radius [m] The basic DLS setup. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. 8580 Macromolecules 34, Notes - NIST Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. Journal Non-Newtonian Fluid Mech., 97, 295-301. Journal Argentine Chemical Society 100 (2013) 25-34. Determination of constants, kM-H and a, from the intrinsic viscosity data, requires a series of monodisperse polymer samples with known molecular weight or a series of polydisperse polymer samples with known viscosity average molecular weights, Mv. Hydrodynamic Properties of Tragacanthin. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. Molecular weight (Mv) and hydrodynamic properties can be calculated from there. Microporation applications for enhancing drug delivery. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). The effect of branching is best recognized from the molecular weight dependencies of []. The dextran was chosen as another branched polysaccharide which, like the others was suspected to show hyperbranched behavior. dextran The [] is connected to the dimensions of the biopolymer molecule isolated in a certain solvent. This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. FITC-dextran solution (600L) was added to the donor chamber. sharing sensitive information, make sure youre on a federal Garcia de la Torre, J.; Carrasco, B. Drug Deliv. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. The Viscosity of Dilute Solutions of Long-Chain Molecules. Webwhittier union high school district superintendent. The hydrodynamic radius (Rh) of individual samples of dextran, polyvinylpyrrolidone, and polyoxyethylene over a range of molecular weights (1,500 Harding, S. E., & Simpkin, N. J. Dextrans in Aqueous Solution. DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. Ubbelohde, Leo. Springer Nature. Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). All authors read and approved the final manuscript. In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. Carbohydrate Polymers 72, 356-360. Human skins (Full-thickness human skin) were supplied by HansBiomed. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining. The value of kM-H in this work decreases with increasing temperature in contrast to published by reference [34] where kM-H increases. Gillespie, T.; Hulme, M. A. In our present study, microporated skin permeated insufficient amount of 20K FITC-dextran, suggesting that enhancement of permeability was unsuccessful with macromolecules of more than 20KM.W.. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). dextran hydrodynamic radius. Although we didnt conduct assay for inflammation, we could not find any inflammation symptoms such as redness, blisters, cracked, and thickening. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Guner, A.; Kibarer, G. (2001). In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. This study was supported by the LG Electronics. Carbohydrate Polymers, 74, 372-378. Alternatively, the value of qM-H can be calculated using numerical methods and other-average molecular weights (Mn, Mw, Mz) according to where c and d are empirical polynomial functions of the exponent a. The receptor chamber was filled with PBS. (1958). Biomaterials, 22, 1891-1898. Clipboard, Search History, and several other advanced features are temporarily unavailable. Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. Pathan IB, Setty CM. Charge: Anionic. Carbohydrate Polymers 88 373-381. For a values from 0-0.5 a rigid sphere is predicted in a ideal solvent, from 0.5-0.8 a random coil in good solvent, and from 0.8-2 a rigid or rod like shape is expected (stiff chain). ; Ross-Murphy, S.B. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during.
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